K562 — IGVFSM8338YZLA

released
{
    "@context": "/terms/",
    "@id": "/in-vitro-systems/IGVFSM8338YZLA/",
    "@type": [
        "InVitroSystem",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "IGVFSM8338YZLA",
    "age": "unknown",
    "aliases": [
        "jay-shendure:CRISPRa_VPR_K562"
    ],
    "audit": {
        "WARNING": [
            {
                "category": "missing files",
                "detail": "Construct library set [IGVFDS7581DNXF](/construct-library-sets/IGVFDS7581DNXF/) has no `files`. File sets are expected to have files.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_no_files",
                "path": "/construct-library-sets/IGVFDS7581DNXF/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011966/",
        "component": "functional characterization"
    },
    "classifications": [
        "cell line"
    ],
    "construct_library_sets": [
        {
            "@id": "/construct-library-sets/IGVFDS7581DNXF/",
            "accession": "IGVFDS7581DNXF",
            "file_set_type": "guide library",
            "status": "released"
        }
    ],
    "creation_timestamp": "2023-12-01T23:55:59.592860+00:00",
    "demultiplexed_to": [],
    "donors": [
        "/human-donors/IGVFDO9208RPQQ/"
    ],
    "file_sets": [],
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/jay-shendure/",
        "title": "Jay Shendure, UW"
    },
    "modifications": [
        {
            "@id": "/crispr-modifications/8b61bce4-105c-435e-9b32-1c8903566a9b/",
            "cas": "dCas9",
            "cas_species": "Streptococcus pyogenes (Sp)",
            "fused_domain": "VP64-p65-Rta (VPR)",
            "modality": "activation",
            "status": "released",
            "summary": "CRISPRa Sp-dCas9-VP64-p65-Rta (VPR)"
        }
    ],
    "multiplexed_in": [],
    "notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\" This sample's modifications originally included Modification /modifications/ec803a15-a3c1-4d14-b1be-be3a5ea1ef8a/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_ec803a15-a3c1-4d14-b1be-be3a5ea1ef8a.",
    "origin_of": [],
    "parts": [
        "/in-vitro-systems/IGVFSM9542NUGS/",
        "/in-vitro-systems/IGVFSM1594ZOQH/"
    ],
    "pooled_in": [],
    "product_id": "HD_dCas9-VPR-005",
    "publications": [
        {
            "@id": "/publications/20fcbdbb-5a47-41c6-b80b-e6dd0e8a8d3e/",
            "publication_identifiers": [
                "doi:10.1101/2023.03.28.534017",
                "doi:10.1038/s41467-024-52490-4"
            ],
            "status": "released"
        }
    ],
    "release_timestamp": "2024-08-13T20:47:07.360648+00:00",
    "sample_terms": [
        {
            "@id": "/sample-terms/EFO_0002067/",
            "status": "released",
            "term_name": "K562"
        }
    ],
    "schema_version": "23",
    "sex": "female",
    "sorted_fractions": [],
    "sources": [
        {
            "@id": "/sources/horizondiscovery/",
            "status": "released",
            "title": "Horizon"
        }
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
        "title": "Beth Martin"
    },
    "summary": "K562 cell line, female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library",
    "taxa": "Homo sapiens",
    "url": "https://horizondiscovery.com/en/engineered-cell-lines/products/dcas9-vpr-stable-cell-line",
    "uuid": "46a68ce2-7095-4b95-bf59-7992f149a535",
    "virtual": false
}