GM25256 (WTC-11) — IGVFSM8317ZTFV

released
{
    "@context": "/terms/",
    "@id": "/in-vitro-systems/IGVFSM8317ZTFV/",
    "@type": [
        "InVitroSystem",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "IGVFSM8317ZTFV",
    "age": "unknown",
    "aliases": [
        "jay-shendure:CRISPRa_VPH_NGN2_WTC11_neurons_10Xlane3"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011966/",
        "component": "functional characterization"
    },
    "cell_fate_change_protocol": "/documents/a9ab6aae-48a0-444a-a609-3addf65d3027/",
    "cellular_sub_pool": "10Xlane3",
    "classifications": [
        "differentiated cell specimen"
    ],
    "construct_library_sets": [
        "/construct-library-sets/IGVFDS7581DNXF/"
    ],
    "creation_timestamp": "2023-12-01T23:56:07.618101+00:00",
    "demultiplexed_to": [],
    "donors": [
        "/human-donors/IGVFDO1756PPKO/"
    ],
    "file_sets": [
        {
            "@id": "/auxiliary-sets/IGVFDS2532AVSC/",
            "accession": "IGVFDS2532AVSC",
            "aliases": [
                "jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_CS1"
            ],
            "lab": {
                "title": "Jay Shendure, UW"
            },
            "status": "released",
            "summary": "gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
        },
        {
            "@id": "/measurement-sets/IGVFDS2385QENN/",
            "accession": "IGVFDS2385QENN",
            "aliases": [
                "jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_exprs"
            ],
            "assay_term": {
                "term_name": "in vitro CRISPR screen using single-cell RNA-seq"
            },
            "lab": {
                "title": "Jay Shendure, UW"
            },
            "status": "released",
            "summary": "scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
        },
        {
            "@id": "/analysis-sets/IGVFDS4021XJLW/",
            "accession": "IGVFDS4021XJLW",
            "aliases": [
                "jay-shendure:analysis_set_CRISPRa_ipscNeurons"
            ],
            "lab": {
                "title": "Jay Shendure, UW"
            },
            "status": "released",
            "summary": "scCRISPR activation screen: differential gene expression quantifications, sparse gene count matrix"
        }
    ],
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/jay-shendure/",
        "title": "Jay Shendure, UW"
    },
    "modifications": [
        {
            "@id": "/crispr-modifications/426a4099-35d9-4c65-8851-5e788a3d4e70/",
            "modality": "activation",
            "status": "released",
            "summary": "CRISPRa Sp-dCas9-VPH"
        }
    ],
    "multiplexed_in": [],
    "notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\" This sample's modifications originally included Modification /modifications/2ca03166-1fc8-49fd-a4f9-42184b949ae5/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_2ca03166-1fc8-49fd-a4f9-42184b949ae5.",
    "origin_of": [],
    "part_of": "/in-vitro-systems/IGVFSM9003EMOQ/",
    "parts": [],
    "pooled_in": [],
    "publications": [
        {
            "@id": "/publications/20fcbdbb-5a47-41c6-b80b-e6dd0e8a8d3e/",
            "publication_identifiers": [
                "doi:10.1101/2023.03.28.534017"
            ]
        }
    ],
    "release_timestamp": "2024-08-13T20:47:21.821275+00:00",
    "sample_terms": [
        {
            "@id": "/sample-terms/EFO_0009747/",
            "term_name": "GM25256 (WTC-11)"
        }
    ],
    "schema_version": "23",
    "sex": "male",
    "sorted_fractions": [],
    "sources": [
        {
            "@id": "/sources/martin-kampmann/",
            "title": "Martin Kampmann, UCSF"
        }
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
        "title": "Beth Martin"
    },
    "summary": "GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library",
    "targeted_sample_term": "/sample-terms/CL_0000540/",
    "taxa": "Homo sapiens",
    "time_post_change": 19,
    "time_post_change_units": "day",
    "uuid": "16d20c93-91f8-4005-ae00-ee855ca6bccc",
    "virtual": false
}