HCT116 — IGVFSM8090YJPJ

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS7846UISG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM4493UPPK FACS bin E for 80-90% expression of KITLG

Aliases

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR4_S107

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR1_S5

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR2_S11

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR3_S101

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS4476RLFR

mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR4_S107
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR1_S5
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR2_S11
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR3_S101

Jesse Engreitz, Stanford

IGVFDS6482AXWW

CRISPR degradation, interference FlowFISH screen targeting KITLG: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_KITLG

Jesse Engreitz, Stanford

IGVFDS7080OXFZ

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR1_S5
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR2_S11
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR3_S101
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR4_S107

Jesse Engreitz, Stanford

IGVFDS9621FJCZ

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets