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HCT116 — IGVFSM7955GCNG

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
  • IGVFDS6351FGNG guide (sgRNA) library targeting accessible genome regions in many genomic loci
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM2018OLYL FACS bin B for 10-20% expression of CCND1
Aliases
jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR1-Rep1_S302
jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR2-Rep1_S308
jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR3-Rep1_S320
jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR4-Rep1_S326

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_CCND1_Auxin6hrs-FF2
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_CCND1
Jesse Engreitz, Stanford
gRNA sequencing
  • jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR1-Rep1_S302
  • jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR2-Rep1_S308
  • jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR3-Rep1_S320
  • jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR4-Rep1_S326
Jesse Engreitz, Stanford
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR1-Rep1_S302
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR2-Rep1_S308
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR3-Rep1_S320
  • jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF2-BinB-PCR4-Rep1_S326
Jesse Engreitz, Stanford

Modifications

2 items
degradation
interference

Treatments

1 item
Term Name
Purpose
Type
Summary
activation
chemical
Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford