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HCT116 — IGVFSM7932USPC
HCT116 — IGVFSM7932USPC
released
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Summary
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Sample Terms
HCT116
Classification
cell line
Sex
male
Originated From Sample
IGVFSM7230EIVE
Age
unknown
Construct Library Sets
IGVFDS1891GTLU
guide (sgRNA) library targeting accessible genome regions in many genomic loci
Taxa
Homo sapiens
Sources
atcc
Aliases
jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO0384OZRW
igvf:donor_of_HCT116
male
Homo sapiens
Sample Parts
2 items
Accession
Type
Sample Terms
Summary
IGVFSM4652TCSQ
InVitroSystem
HCT116
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
IGVFSM8653GBIO
InVitroSystem
HCT116
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Modifications
2 items
Summary
Modality
Activation
CRISPRi Sp-dCas9-KOX1-KRAB
interference
inactive AID system targeting RAD21
degradation
Documents
2 items
Page
Description
Type
Download
Preview
Generation of inducible CRISPRi line
plasmid map
addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Download addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Download addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Generation of inducible CRISPRi line
plasmid map
takara#631363_pLVX-EF1alpha-Tet3G.pdf
Download takara#631363_pLVX-EF1alpha-Tet3G.pdf
Download takara#631363_pLVX-EF1alpha-Tet3G.pdf
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford