HCT116 — IGVFSM7863HHJP

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Sources
Sorted From Sample
IGVFSM3580KDPA FACS bin B for 10-20% expression of CCND1
Aliases
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR1_S74
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR2_S170
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR3_S266
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR4_S362

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR1_S74
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR2_S170
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR3_S266
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR4_S362
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_CCND1_pilot
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_PILOT_CCND1-Auxin0hrs-FF1
Jesse Engreitz, Stanford
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR1_S74
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR2_S170
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR3_S266
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR4_S362
Jesse Engreitz, Stanford

Modifications

2 items

Documents

Attribution