HCT116 — IGVFSM7735WAOD

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM6219XAXK FACS bin D for 55-65% expression of CCND1

Aliases

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR1_S4

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR2_S100

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR3_S196

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR4_S292

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS2620FWYA

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR1_S4
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR2_S100
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR3_S196
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR4_S292

Jesse Engreitz, Stanford

IGVFDS2982SJGW

CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_CCND1_pilot

Jesse Engreitz, Stanford

IGVFDS5146ALYO

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR1_S4
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR2_S100
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR3_S196
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR4_S292

Jesse Engreitz, Stanford

IGVFDS5877TZTD

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets