HCT116 — IGVFSM7299CMAO

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM3220YBCR FACS bin C for 35-45% expression of KITLG

Aliases

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR1_S63

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR2_S69

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR3_S159

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR4_S165

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS3075NNHV

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR1_S63
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR2_S69
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR3_S159
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR4_S165

Jesse Engreitz, Stanford

IGVFDS6482AXWW

CRISPR degradation, interference FlowFISH screen targeting KITLG: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_KITLG

Jesse Engreitz, Stanford

IGVFDS8710ZSOZ

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR1_S63
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR2_S69
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR3_S159
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR4_S165

Jesse Engreitz, Stanford

IGVFDS8781ZUPM

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets