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HCT116 — IGVFSM7258NLNH

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM6328ZXHI FACS bin C for 35-45% expression of MYC
Aliases
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep1_S27
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep2_S82
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep2_S88
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep1_S39
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep2_S94
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep1_S45
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep2_S100

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep1_S27
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep2_S82
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep2_S88
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep1_S39
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep2_S94
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep1_S45
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep2_S100
Jesse Engreitz, Stanford
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep1_S27
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep2_S82
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep2_S88
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep1_S39
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep2_S94
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep1_S45
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep2_S100
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting MYC: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_MYC
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin0hrs-FF2
Jesse Engreitz, Stanford

Modifications

2 items
interference
degradation

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford