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HCT116 — IGVFSM7141IWGF

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM0637ZUKN FACS bin F for 80-100% expression of KITLG
Aliases
jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR1_S30
jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR2_S36
jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR3_S126
jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR4_S132

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin0hrs-FF3
Jesse Engreitz, Stanford
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR1_S30
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR2_S36
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR3_S126
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR4_S132
Jesse Engreitz, Stanford
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR1_S30
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR2_S36
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR3_S126
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR4_S132
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting KITLG: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_KITLG
Jesse Engreitz, Stanford

Modifications

2 items
interference
degradation

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford