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Jurkat — IGVFSM6964ODEE

released
Summary
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
  • IGVFDS8183QSJO guide (sgRNA) library targeting transcription start sites in IL2RA
Taxa
Homo sapiens
Sources
stem-cell-technologies
Sorted From Sample
IGVFSM6721RTTV FACS bin for 90-100% expression of IL2RA
Aliases
charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-high-bin-rep2
Publications
Attribution
Charles Gersbach, Duke

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

File Sets

3 items
gRNA sequencing
  • charles-gersbach:jurkats-auxiliary-set-crispra-vp64x2-il2ra-high-bin-rep2
Charles Gersbach, Duke
CRISPR activation FACS screen targeting IL2RA: alignments, fold change over control, guide quantifications
  • charles-gersbach:jurkats-analysis-set-crispra-vp64x2-il2ra
Charles Gersbach, Duke
CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
  • charles-gersbach:jurkats-measurement-set-crispra-vp64x2-il2ra-high-bin-rep2
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
Activation
activation