HCT116 — IGVFSM6932DXSV

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS6351FGNG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM7006DNJX FACS bin F for 80-100% expression of CCND1

Aliases

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR1-Rep1_S234

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR2-Rep1_S240

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR3-Rep1_S276

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR4-Rep1_S282

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS1549RKIJ

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

jesse-engreitz:auxiliary_sortparams_CRUDO_CCND1_Auxin0hrs-FF2

Jesse Engreitz, Stanford

IGVFDS1569RNPD

CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_CCND1

Jesse Engreitz, Stanford

IGVFDS3113JRZB

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR1-Rep1_S234
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR2-Rep1_S240
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR3-Rep1_S276
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR4-Rep1_S282

Jesse Engreitz, Stanford

IGVFDS3504GXZA

mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR1-Rep1_S234
jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR2-Rep1_S240
jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR3-Rep1_S276
jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR4-Rep1_S282

Jesse Engreitz, Stanford