HCT116 — IGVFSM6928JPHO

released
{
    "@context": "/terms/",
    "@id": "/in-vitro-systems/IGVFSM6928JPHO/",
    "@type": [
        "InVitroSystem",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "IGVFSM6928JPHO",
    "age": "unknown",
    "aliases": [
        "jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR1-Rep1_S136",
        "jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR2-Rep1_S142",
        "jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR3-Rep1_S190",
        "jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR4-Rep1_S196"
    ],
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Construct library set [IGVFDS1891GTLU](/construct-library-sets/IGVFDS1891GTLU/) has sequence file(s) [IGVFFI0445AYJF](/sequence-files/IGVFFI0445AYJF/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/construct-library-sets/IGVFDS1891GTLU/"
            }
        ],
        "NOT_COMPLIANT": [
            {
                "category": "missing analysis",
                "detail": "Construct library set [IGVFDS1891GTLU](/construct-library-sets/IGVFDS1891GTLU/) is a raw data set with files, but is not listed in any `input_file_sets` for any analysis sets. Raw data sets with files are expected to be associated with at least one analysis set.",
                "level": 50,
                "level_name": "NOT_COMPLIANT",
                "name": "audit_input_for",
                "path": "/construct-library-sets/IGVFDS1891GTLU/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "classifications": [
        "cell line"
    ],
    "construct_library_sets": [
        {
            "@id": "/construct-library-sets/IGVFDS1891GTLU/",
            "accession": "IGVFDS1891GTLU",
            "file_set_type": "guide library",
            "status": "released"
        }
    ],
    "creation_timestamp": "2024-05-09T23:20:53.200377+00:00",
    "demultiplexed_to": [],
    "documents": [
        "/documents/a1a19741-90cc-46ea-b90c-96c4a4b70d8e/",
        "/documents/b0663905-6249-42ea-9937-9b2f645c787d/"
    ],
    "donors": [
        "/human-donors/IGVFDO0384OZRW/"
    ],
    "file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS2315UDUR/",
            "accession": "IGVFDS2315UDUR",
            "aliases": [
                "jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR1-Rep1_S136",
                "jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR2-Rep1_S142",
                "jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR3-Rep1_S190",
                "jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR4-Rep1_S196"
            ],
            "file_set_type": "experimental data",
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "preferred_assay_title": "CRISPR FlowFISH screen",
            "status": "released",
            "summary": "mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
        },
        {
            "@id": "/auxiliary-sets/IGVFDS9525OEUV/",
            "accession": "IGVFDS9525OEUV",
            "aliases": [
                "jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR1-Rep1_S136",
                "jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR2-Rep1_S142",
                "jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR3-Rep1_S190",
                "jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR4-Rep1_S196"
            ],
            "file_set_type": "gRNA sequencing",
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "status": "released",
            "summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
        },
        {
            "@id": "/auxiliary-sets/IGVFDS9190KTAF/",
            "accession": "IGVFDS9190KTAF",
            "aliases": [
                "jesse-engreitz:auxiliary_sortparams_CRUDO_FAM3C_Auxin6hrs-FF1"
            ],
            "file_set_type": "cell sorting",
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "status": "released",
            "summary": "cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets"
        },
        {
            "@id": "/analysis-sets/IGVFDS5239VQTN/",
            "accession": "IGVFDS5239VQTN",
            "aliases": [
                "jesse-engreitz:analysis_set_CRUDO_FAM3C"
            ],
            "file_set_type": "principal analysis",
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "status": "in progress",
            "summary": "CRISPR degradation, interference FlowFISH screen targeting FAM3C: differential element quantifications"
        }
    ],
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "modifications": [
        {
            "@id": "/degron-modifications/95081d1b-08b4-49ed-b324-e53fc43d8456/",
            "degron_system": "AID",
            "modality": "degradation",
            "status": "released",
            "summary": "AID system targeting RAD21",
            "tagged_proteins": [
                {
                    "@id": "/genes/ENSG00000164754/",
                    "status": "released",
                    "summary": "RAD21 - ENSG00000164754 (Homo sapiens)",
                    "symbol": "RAD21"
                }
            ]
        },
        {
            "@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
            "cas": "dCas9",
            "cas_species": "Streptococcus pyogenes (Sp)",
            "fused_domain": "KOX1-KRAB",
            "modality": "interference",
            "status": "released",
            "summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
        }
    ],
    "multiplexed_in": [],
    "notes": "This sample's modifications originally included Modification /modifications/56d6a4f4-5fa4-4989-890e-aafb032f1e7d/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_56d6a4f4-5fa4-4989-890e-aafb032f1e7d.",
    "origin_of": [],
    "parts": [],
    "pooled_in": [],
    "release_timestamp": "2024-07-10T19:41:05.421396+00:00",
    "sample_terms": [
        {
            "@id": "/sample-terms/EFO_0002824/",
            "status": "released",
            "term_name": "HCT116"
        }
    ],
    "schema_version": "23",
    "sex": "male",
    "sorted_fractions": [],
    "sorted_from": {
        "@id": "/in-vitro-systems/IGVFSM4608EHIU/",
        "accession": "IGVFSM4608EHIU",
        "status": "released"
    },
    "sorted_from_detail": "FACS bin D for 55-65% expression of FAM3C",
    "sources": [
        {
            "@id": "/sources/atcc/",
            "status": "released",
            "title": "ATCC"
        }
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
        "title": "Chongyon Park"
    },
    "summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
    "taxa": "Homo sapiens",
    "treatments": [
        {
            "@id": "/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/",
            "depletion": false,
            "purpose": "activation",
            "status": "released",
            "treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid",
            "treatment_type": "chemical"
        }
    ],
    "uuid": "eb41e091-7a54-4f79-9894-d2b16213de01",
    "virtual": false
}