THP-1 — IGVFSM6883YPDS

{
    "@context": "/terms/",
    "@id": "/in-vitro-systems/IGVFSM6883YPDS/",
    "@type": [
        "InVitroSystem",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "IGVFSM6883YPDS",
    "age": "unknown",
    "aliases": [
        "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCCA-THP1-BioRep1"
    ],
    "audit": {
        "ERROR": [
            {
                "category": "inconsistent parent sample",
                "detail": "In vitro system [IGVFSM6883YPDS](/in-vitro-systems/IGVFSM6883YPDS/) has metadata properties (`construct_library_sets`) inconsistent with its associated parent sample [IGVFSM3498MEQA](/in-vitro-systems/IGVFSM3498MEQA/). Samples that are sorted from or part of a parent sample are expected to share most of the parent's metadata properties.",
                "level": 60,
                "level_name": "ERROR",
                "name": "audit_sample_sorted_from_parent_child_check",
                "path": "/in-vitro-systems/IGVFSM6883YPDS/"
            }
        ],
        "NOT_COMPLIANT": [
            {
                "category": "missing plasmid map",
                "detail": "Construct library set [IGVFDS9097TEUZ](/construct-library-sets/IGVFDS9097TEUZ/) does not have a plasmid map attached in `documents`. Construct library sets are expected to be associated with a plasmid map document.",
                "level": 50,
                "level_name": "NOT_COMPLIANT",
                "name": "audit_construct_library_set_plasmid_map",
                "path": "/construct-library-sets/IGVFDS9097TEUZ/"
            },
            {
                "category": "missing guide RNA sequences",
                "detail": "Construct library set [IGVFDS9097TEUZ](/construct-library-sets/IGVFDS9097TEUZ/) has no `integrated_content_files`. Guide libraries are expected to link to an integrated content file of guide RNA sequences.",
                "level": 50,
                "level_name": "NOT_COMPLIANT",
                "name": "audit_integrated_content_files",
                "path": "/construct-library-sets/IGVFDS9097TEUZ/"
            }
        ],
        "WARNING": [
            {
                "category": "missing files",
                "detail": "Construct library set [IGVFDS9097TEUZ](/construct-library-sets/IGVFDS9097TEUZ/) has no `files`. File sets are expected to have files.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_no_files",
                "path": "/construct-library-sets/IGVFDS9097TEUZ/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "classifications": [
        "cell line"
    ],
    "construct_library_sets": [
        {
            "@id": "/construct-library-sets/IGVFDS9097TEUZ/",
            "accession": "IGVFDS9097TEUZ",
            "file_set_type": "guide library",
            "status": "preview"
        }
    ],
    "creation_timestamp": "2024-12-10T22:23:53.761547+00:00",
    "demultiplexed_to": [],
    "donors": [
        "/human-donors/IGVFDO7359LVPI/"
    ],
    "file_sets": [],
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "modifications": [
        {
            "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
            "cas": "nCas9",
            "cas_species": "Streptococcus pyogenes (Sp)",
            "fused_domain": "M-MLV RT (PE2)",
            "modality": "prime editing",
            "status": "released",
            "summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
        }
    ],
    "multiplexed_in": [],
    "origin_of": [],
    "part_of": "/in-vitro-systems/IGVFSM3498MEQA/",
    "parts": [
        "/in-vitro-systems/IGVFSM0430AXWK/",
        "/in-vitro-systems/IGVFSM1539RRGS/"
    ],
    "pooled_in": [],
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "sample_terms": [
        {
            "@id": "/sample-terms/EFO_0001253/",
            "status": "released",
            "term_name": "THP-1"
        }
    ],
    "schema_version": "23",
    "sex": "male",
    "sorted_fractions": [],
    "sources": [
        {
            "@id": "/sources/atcc/",
            "status": "released",
            "title": "ATCC"
        }
    ],
    "status": "preview",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "summary": "THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
    "taxa": "Homo sapiens",
    "uuid": "1f08a84d-a30b-4eed-a7a3-99a0b4fc2487",
    "virtual": false
}