HCT116 — IGVFSM6010ONZP

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM8770MGOE FACS bin F for 80-100% expression of SSFA2

Aliases

jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR1-Rep1_S30

jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR2-Rep1_S36

jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR3-Rep1_S84

jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR4-Rep1_S90

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS2031CTSP

CRISPR degradation, interference FlowFISH screen targeting ITPRID2: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_SSFA2

Jesse Engreitz, Stanford

IGVFDS4138DUVP

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR1-Rep1_S30
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR2-Rep1_S36
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR3-Rep1_S84
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR4-Rep1_S90

Jesse Engreitz, Stanford

IGVFDS6020CDHF

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets