TeloHAEC — IGVFSM5826EWDE

released
{
    "@context": "/terms/",
    "@id": "/in-vitro-systems/IGVFSM5826EWDE/",
    "@type": [
        "InVitroSystem",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "IGVFSM5826EWDE",
    "age": "23",
    "age_units": "year",
    "aliases": [
        "jesse-engreitz:in-vitro-system_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_Rep4_bin4"
    ],
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "NTR term ID",
                "detail": "Sample term for [NTR_0000753](/sample-terms/NTR_0000753/) has been newly requested. Ontology terms with a newly requested term ID are expected to have it updated following its addition to the appropriate ontology database.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_ntr_term_id",
                "path": "/sample-terms/NTR_0000753/"
            },
            {
                "category": "mismatched status",
                "detail": "Released construct library set [IGVFDS7621RRLM](/construct-library-sets/IGVFDS7621RRLM/) has deleted subobject tabular file [IGVFFI1837MRQJ](/tabular-files/IGVFFI1837MRQJ/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/construct-library-sets/IGVFDS7621RRLM/"
            }
        ],
        "WARNING": [
            {
                "category": "missing files",
                "detail": "Construct library set [IGVFDS7621RRLM](/construct-library-sets/IGVFDS7621RRLM/) has no `files`. File sets are expected to have files.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_no_files",
                "path": "/construct-library-sets/IGVFDS7621RRLM/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "classifications": [
        "cell line"
    ],
    "construct_library_sets": [
        {
            "@id": "/construct-library-sets/IGVFDS7621RRLM/",
            "accession": "IGVFDS7621RRLM",
            "file_set_type": "guide library",
            "status": "released"
        }
    ],
    "creation_timestamp": "2024-01-12T01:29:45.393473+00:00",
    "demultiplexed_to": [],
    "donors": [
        "/human-donors/IGVFDO3459DFTW/"
    ],
    "file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS2419JZMR/",
            "accession": "IGVFDS2419JZMR",
            "aliases": [
                "jesse-engreitz:measurement_set_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_Rep4_bin4"
            ],
            "file_set_type": "experimental data",
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "preferred_assay_title": "CRISPR FlowFISH screen",
            "status": "released",
            "summary": "CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus"
        },
        {
            "@id": "/auxiliary-sets/IGVFDS8749RHTV/",
            "accession": "IGVFDS8749RHTV",
            "aliases": [
                "jesse-engreitz:auxiliary_set_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_Rep4_bin4"
            ],
            "file_set_type": "gRNA sequencing",
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "status": "released",
            "summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus"
        },
        {
            "@id": "/analysis-sets/IGVFDS9053MHCS/",
            "accession": "IGVFDS9053MHCS",
            "aliases": [
                "jesse-engreitz:analysis_set_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_analysis"
            ],
            "file_set_type": "principal analysis",
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "status": "released",
            "summary": "CRISPR interference FlowFISH screen targeting TLNRD1"
        }
    ],
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "lower_bound_age": 23,
    "lower_bound_age_in_hours": 201480,
    "modifications": [
        {
            "@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
            "cas": "dCas9",
            "cas_species": "Streptococcus pyogenes (Sp)",
            "fused_domain": "KOX1-KRAB",
            "modality": "interference",
            "status": "released",
            "summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
        }
    ],
    "multiplexed_in": [],
    "notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\" This sample's modifications originally included Modification /modifications/2ba01755-df67-453b-b047-f7cdba236665/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_2ba01755-df67-453b-b047-f7cdba236665.",
    "origin_of": [],
    "parts": [],
    "pooled_in": [],
    "release_timestamp": "2024-02-07T19:13:54.249801+00:00",
    "sample_terms": [
        {
            "@id": "/sample-terms/NTR_0000753/",
            "status": "released",
            "term_name": "TeloHAEC"
        }
    ],
    "schema_version": "23",
    "sex": "female",
    "sorted_fractions": [],
    "sorted_from": {
        "@id": "/in-vitro-systems/IGVFSM9485EHBX/",
        "accession": "IGVFSM9485EHBX",
        "status": "released"
    },
    "sorted_from_detail": "FACS bin for 76-100% expression of TLNRD1",
    "sources": [
        {
            "@id": "/sources/atcc/",
            "status": "released",
            "title": "ATCC"
        }
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/4b2083b4-24c1-41d8-9daa-e79299f00d6e/",
        "title": "Ronghao Zhou"
    },
    "summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
    "taxa": "Homo sapiens",
    "upper_bound_age": 23,
    "upper_bound_age_in_hours": 201480,
    "url": "https://www.atcc.org/products/crl-4052",
    "uuid": "703f65c8-aa92-4992-a648-0815ba1183fd",
    "virtual": false
}