{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM5659PDUJ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5659PDUJ",
"age": "unknown",
"aliases": [
"jay-shendure:CRISPRa_singletonvalidations_K562_rep1"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS9531XJKW](/construct-library-sets/IGVFDS9531XJKW/) has in progress subobject tabular file [IGVFFI9694NCRL](/tabular-files/IGVFFI9694NCRL/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS9531XJKW/"
}
]
},
"award": {
"@id": "/awards/HG011966/",
"component": "functional characterization"
},
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS9531XJKW/",
"accession": "IGVFDS9531XJKW",
"file_set_type": "guide library",
"status": "released"
}
],
"creation_timestamp": "2024-10-17T20:52:03.997852+00:00",
"demultiplexed_to": [],
"donors": [
{
"@id": "/human-donors/IGVFDO9208RPQQ/",
"accession": "IGVFDO9208RPQQ",
"status": "released"
}
],
"embryonic": false,
"file_sets": [
{
"@id": "/measurement-sets/IGVFDS9413OZYX/",
"accession": "IGVFDS9413OZYX",
"aliases": [
"jay-shendure:CRISPRa_singlevalidations_K562_ASIC1_rep1"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jay Shendure, UW"
},
"preferred_assay_title": "RNA-seq",
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS8019CMKZ/",
"accession": "IGVFDS8019CMKZ",
"aliases": [
"jay-shendure:CRISPRa_singlevalidations_K562_ANXA1_rep1"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jay Shendure, UW"
},
"preferred_assay_title": "RNA-seq",
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS9143FEVA/",
"accession": "IGVFDS9143FEVA",
"aliases": [
"jay-shendure:CRISPRa_singlevalidations_K562_FOXP1_rep1"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jay Shendure, UW"
},
"preferred_assay_title": "RNA-seq",
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS0448BPQQ/",
"accession": "IGVFDS0448BPQQ",
"aliases": [
"jay-shendure:CRISPRa_singlevalidations_K562_TBR1_rep1"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jay Shendure, UW"
},
"preferred_assay_title": "RNA-seq",
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS8662QDUM/",
"accession": "IGVFDS8662QDUM",
"aliases": [
"jay-shendure:CRISPRa_singlevalidations_K562_HMGA1_rep1"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jay Shendure, UW"
},
"preferred_assay_title": "RNA-seq",
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS4232HUIF/",
"accession": "IGVFDS4232HUIF",
"aliases": [
"jay-shendure:CRISPRa_singlevalidations_K562_BCL11A_rep1"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jay Shendure, UW"
},
"preferred_assay_title": "RNA-seq",
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS0074FRYO/",
"accession": "IGVFDS0074FRYO",
"aliases": [
"jay-shendure:CRISPRa_singlevalidations_K562_DNMT3B_rep1"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jay Shendure, UW"
},
"preferred_assay_title": "RNA-seq",
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS1813PQFV/",
"accession": "IGVFDS1813PQFV",
"aliases": [
"jay-shendure:CRISPRa_singlevalidations_K562_TCF4_rep1"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jay Shendure, UW"
},
"preferred_assay_title": "RNA-seq",
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/analysis-sets/IGVFDS3251QBOZ/",
"accession": "IGVFDS3251QBOZ",
"aliases": [
"jay-shendure:analysis_set_singletonvalidation"
],
"file_set_type": "principal analysis",
"lab": {
"title": "Jay Shendure, UW"
},
"status": "released",
"summary": "CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
}
],
"institutional_certificates": [
{
"@id": "/institutional-certificates/40798097-3324-47f9-aa9b-3598e01224e4/",
"certificate_identifier": "IP118-02",
"controlled_access": false,
"data_use_limitation_summary": "No limitations",
"status": "released"
}
],
"lab": {
"@id": "/labs/jay-shendure/",
"title": "Jay Shendure, UW"
},
"modifications": [
{
"@id": "/crispr-modifications/ee99bf66-2a5a-4ec6-8380-9434b259afd1/",
"cas": "dCas9",
"cas_species": "Streptococcus pyogenes (Sp)",
"fused_domain": "VP64",
"modality": "activation",
"status": "released",
"summary": "CRISPRa Sp-dCas9-VP64"
}
],
"multiplexed_in": [],
"nucleic_acid_delivery": "nucleofection",
"origin_of": [],
"parts": [],
"pooled_in": [],
"publications": [
{
"@id": "/publications/20fcbdbb-5a47-41c6-b80b-e6dd0e8a8d3e/",
"publication_identifiers": [
"doi:10.1038/s41467-024-52490-4"
],
"status": "released"
}
],
"release_timestamp": "2025-05-29T21:02:01.076673+00:00",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002067/",
"status": "released",
"term_name": "K562"
}
],
"schema_version": "28",
"sex": "female",
"sorted_fractions": [],
"sources": [
{
"@id": "/sources/horizondiscovery/",
"status": "released",
"title": "Horizon"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
"title": "Beth Martin"
},
"summary": "Homo sapiens (female) K562 cell line modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library",
"taxa": "Homo sapiens",
"uuid": "b3446a16-4475-4069-82c6-7a850560ea38",
"virtual": false
}