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H9 — IGVFSM5657YKPN

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This object is in preview status. Metadata is subject to change until this object is released.
Summary
Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW271) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Sample Terms
Classification
differentiated cell specimen
Sex
female
Cellular Sub Pool
subpool_LW271
Originated From Sample
IGVFSM7411AQVC
Age
unknown
Part of Sample
IGVFSM8305VDSK
Targeted Sample Term
cardiac muscle cell
Time Post Change
12 day
Cell Fate Change Protocol
Protocol-hPSC-CM-differentiation-protocol-DRAFTv2.1.pdf
Construct Library Sets
  • IGVFDS9629YZLO guide (sgRNA) library targeting accessible genome regions in many genomic loci
Taxa
Homo sapiens
Sources
Gary Hon, UT
Aliases
gary-hon:differentiated_H9_shen_construct_sgRNA_Lenti_LW271
Attribution
Gary Hon, UT

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_H9
female
Homo sapiens

File Sets

4 items
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:CM_Pilot_2_Mini_sgRNA_Lenti_LW271
Gary Hon, UT
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:CM_Pilot_2_Mini_HTO_Lenti_LW271
Gary Hon, UT
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:H9_shen_construct_sgRNA_Lenti_PrimaryAnalysis
Gary Hon, UT
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:CM_Pilot_2_Mini_Lenti_LW271
Gary Hon, UT

Modifications

1 item
Summary
Modality
Activation
interference

Documents

1 item
Page
Description
Type
Download
Standardized File Format
Preview
Stem Cell DIfferentiation Protocol
cell fate change protocol