HCT116 — IGVFSM5255ABUW

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS6351FGNG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM2423BKRB FACS bin B for 10-20% expression of CCND1

Aliases

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR1-Rep1_S242

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR2-Rep1_S248

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR3-Rep1_S284

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR4-Rep1_S290

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS0051MRCH

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR1-Rep1_S242
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR2-Rep1_S248
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR3-Rep1_S284
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR4-Rep1_S290

Jesse Engreitz, Stanford

IGVFDS1569RNPD

CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_CCND1

Jesse Engreitz, Stanford

IGVFDS5572SQRK

mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR1-Rep1_S242
jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR2-Rep1_S248
jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR3-Rep1_S284
jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR4-Rep1_S290

Jesse Engreitz, Stanford

IGVFDS6842FAIJ

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets