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HCT116 — IGVFSM5079ZTJX

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
  • IGVFDS0392OWNZ guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM3580KDPA FACS bin C for 35-45% expression of CCND1
Aliases
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR1_S75
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR2_S171
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR3_S267
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR4_S363

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR1_S75
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR2_S171
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR3_S267
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR4_S363
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_CCND1_pilot
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_PILOT_CCND1-Auxin0hrs-FF1
Jesse Engreitz, Stanford
gRNA sequencing
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR1_S75
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR2_S171
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR3_S267
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR4_S363
Jesse Engreitz, Stanford

Modifications

2 items
interference
degradation

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford