HCT116 — IGVFSM4674ZTIR

released

Summary

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS0392OWNZ guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM3580KDPA FACS bin F for 80-100% expression of CCND1

Aliases

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR1_S78

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR2_S174

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR3_S270

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR4_S366

Attribution

Jesse Engreitz, Stanford

(

HG011972 — Jesse Engreitz

)

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS2982SJGW

CRISPR mixed FlowFISH screen targeting CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_CCND1_pilot

Jesse Engreitz, Stanford

IGVFDS3029MTKI

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR1_S78
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR2_S174
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR3_S270
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR4_S366

Jesse Engreitz, Stanford

IGVFDS4315AMOI

mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR1_S78
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR2_S174
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR3_S270
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR4_S366

Jesse Engreitz, Stanford

IGVFDS5292OCBC

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets