HCT116 — IGVFSM4441KRKU

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM3220YBCR FACS bin E for 80-90% expression of KITLG

Aliases

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR1_S65

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR2_S71

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR3_S161

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR4_S167

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS0548YILY

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR1_S65
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR2_S71
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR3_S161
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR4_S167

Jesse Engreitz, Stanford

IGVFDS1005OGXR

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR1_S65
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR2_S71
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR3_S161
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR4_S167

Jesse Engreitz, Stanford

IGVFDS6482AXWW

CRISPR degradation, interference FlowFISH screen targeting KITLG: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_KITLG

Jesse Engreitz, Stanford

IGVFDS8781ZUPM

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets