HCT116 — IGVFSM3569TFCR

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS1891GTLU guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM4652TCSQ FACS bin B for 10-20% expression of FAM3C

Aliases

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR1-Rep1_S122

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR2-Rep1_S128

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR3-Rep1_S176

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR4-Rep1_S182

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS0713EITK

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 2 more measurement sets

jesse-engreitz:auxiliary_sortparams_CRUDO_FAM3C_Auxin0hrs-FF2

Jesse Engreitz, Stanford

IGVFDS4010VNGF

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR1-Rep1_S122
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR2-Rep1_S128
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR3-Rep1_S176
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR4-Rep1_S182

Jesse Engreitz, Stanford

IGVFDS5239VQTN

CRISPR degradation, interference FlowFISH screen targeting FAM3C: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_FAM3C

Jesse Engreitz, Stanford

IGVFDS6663FDTV

mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR1-Rep1_S122
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR2-Rep1_S128
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR3-Rep1_S176
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR4-Rep1_S182

Jesse Engreitz, Stanford