{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM3472QIPU/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3472QIPU",
"age": "23",
"age_units": "year",
"aliases": [
"jesse-engreitz:in-vitro-system_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH"
],
"audit": {
"ERROR": [
{
"category": "inconsistent parent sample",
"detail": "In vitro system [IGVFSM3472QIPU](/in-vitro-systems/IGVFSM3472QIPU/) has metadata properties (`construct_library_sets`) inconsistent with its associated parent sample [IGVFSM6912FONC](/in-vitro-systems/IGVFSM6912FONC/). Samples that are sorted from or part of a parent sample are expected to share most of the parent's metadata properties.",
"level": 60,
"level_name": "ERROR",
"name": "audit_sample_sorted_from_parent_child_check",
"path": "/in-vitro-systems/IGVFSM3472QIPU/"
}
],
"INTERNAL_ACTION": [
{
"category": "NTR term ID",
"detail": "Sample term for [NTR_0000753](/sample-terms/NTR_0000753/) has been newly requested. Ontology terms with a newly requested term ID are expected to have it updated following its addition to the appropriate ontology database.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_ntr_term_id",
"path": "/sample-terms/NTR_0000753/"
},
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS7621RRLM](/construct-library-sets/IGVFDS7621RRLM/) has deleted subobject tabular file [IGVFFI1837MRQJ](/tabular-files/IGVFFI1837MRQJ/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS7621RRLM/"
}
],
"WARNING": [
{
"category": "missing files",
"detail": "Construct library set [IGVFDS7621RRLM](/construct-library-sets/IGVFDS7621RRLM/) has no `files`. File sets are expected to have files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_no_files",
"path": "/construct-library-sets/IGVFDS7621RRLM/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS7621RRLM/",
"accession": "IGVFDS7621RRLM",
"file_set_type": "guide library"
}
],
"creation_timestamp": "2024-01-12T23:08:00.924893+00:00",
"demultiplexed_to": [],
"donors": [
"/human-donors/IGVFDO3459DFTW/"
],
"file_sets": [],
"institutional_certificates": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"lower_bound_age": 23,
"lower_bound_age_in_hours": 201480,
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"cas": "dCas9",
"cas_species": "Streptococcus pyogenes (Sp)",
"fused_domain": "KOX1-KRAB",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"multiplexed_in": [],
"notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\" This sample's modifications originally included Modification /modifications/2ba01755-df67-453b-b047-f7cdba236665/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_2ba01755-df67-453b-b047-f7cdba236665.",
"origin_of": [],
"part_of": "/in-vitro-systems/IGVFSM6912FONC/",
"parts": [
"/in-vitro-systems/IGVFSM2326VRBK/",
"/in-vitro-systems/IGVFSM6404DFQW/",
"/in-vitro-systems/IGVFSM2880OLBN/",
"/in-vitro-systems/IGVFSM9485EHBX/"
],
"pooled_in": [],
"release_timestamp": "2024-02-07T19:14:19.922095+00:00",
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"schema_version": "23",
"sex": "female",
"sorted_fractions": [],
"sources": [
{
"@id": "/sources/atcc/",
"title": "ATCC"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/4b2083b4-24c1-41d8-9daa-e79299f00d6e/",
"title": "Ronghao Zhou"
},
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens",
"upper_bound_age": 23,
"upper_bound_age_in_hours": 201480,
"url": "https://www.atcc.org/products/crl-4052",
"uuid": "0fb2abcb-b66a-4e21-b81e-f4d8b3c5b05e",
"virtual": false
}