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Jurkat — IGVFSM3244YHOI

released
Summary
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Classification
cell line
Sex
unspecified
Age
unknown
Taxa
Homo sapiens
Sources
stem-cell-technologies
Sorted From Sample
IGVFSM6606RDWT FACS bin for 90-100% expression of IL2RA
Aliases
charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-high-bin-rep3
Publications

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:jurkat-tcells-donor-6
unspecified
Homo sapiens

File Sets

5 items
Homo sapiens GRCh38 custom guide RNAs
  • charles-gersbach:jurkat-il2ra-grnas-set
Charles Gersbach, Duke
CRISPR activation FACS screen targeting IL2RA: alignments
  • charles-gersbach:jurkats-intermediate-analysis-crispra-vp64x2-il2ra
Charles Gersbach, Duke
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
  • charles-gersbach:jurkats-measurement-set-crispra-vp64x2-il2ra-high-bin-rep3
Charles Gersbach, Duke
CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications
  • charles-gersbach:jurkats-analysis-set-crispra-vp64x2-il2ra
Charles Gersbach, Duke
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
  • charles-gersbach:jurkats-auxiliary-set-crispra-vp64x2-il2ra-high-bin-rep3
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
activation

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke