{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM3012RMVA/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3012RMVA",
"age": "unknown",
"aliases": [
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR1_Rep1_S140",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR1_Rep2_S195",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR2_Rep1_S146",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR2_Rep2_S201",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR3_Rep1_S152",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR3_Rep2_S207",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR4_Rep1_S158",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR4_Rep2_S213"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM3012RMVA](/in-vitro-systems/IGVFSM3012RMVA/) has in progress subobject degron modification [95081d1b-08b4-49ed-b324-e53fc43d8456](/degron-modifications/95081d1b-08b4-49ed-b324-e53fc43d8456/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM3012RMVA/"
},
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM3012RMVA](/in-vitro-systems/IGVFSM3012RMVA/) has in progress subobject treatment [d35868d1-c45b-49ae-8ff3-a9c0c22551c2](/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM3012RMVA/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"classifications": [
"cell line"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS7857SYCK/"
],
"creation_timestamp": "2024-05-20T23:12:48.480023+00:00",
"demultiplexed_to": [],
"documents": [
"/documents/a1a19741-90cc-46ea-b90c-96c4a4b70d8e/",
"/documents/b0663905-6249-42ea-9937-9b2f645c787d/"
],
"donors": [
"/human-donors/IGVFDO0384OZRW/"
],
"file_sets": [
{
"@id": "/measurement-sets/IGVFDS1564ZVKC/",
"accession": "IGVFDS1564ZVKC",
"aliases": [
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR1_Rep1_S140",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR1_Rep2_S195",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR2_Rep1_S146",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR2_Rep2_S201",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR3_Rep1_S152",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR3_Rep2_S207",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR4_Rep1_S158",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR4_Rep2_S213"
],
"assay_term": {
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
{
"@id": "/auxiliary-sets/IGVFDS6660JNZU/",
"accession": "IGVFDS6660JNZU",
"aliases": [
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR1_Rep1_S140",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR1_Rep2_S195",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR2_Rep1_S146",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR2_Rep2_S201",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR3_Rep1_S152",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR3_Rep2_S207",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR4_Rep1_S158",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinF-PCR4_Rep2_S213"
],
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
{
"@id": "/auxiliary-sets/IGVFDS5394TNHK/",
"accession": "IGVFDS5394TNHK",
"aliases": [
"jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin6hrs-FF2"
],
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets"
}
],
"institutional_certificates": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"modifications": [
{
"@id": "/degron-modifications/95081d1b-08b4-49ed-b324-e53fc43d8456/",
"modality": "degradation",
"status": "in progress",
"summary": "AID system targeting RAD21"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"multiplexed_in": [],
"notes": "This sample's modifications originally included Modification /modifications/56d6a4f4-5fa4-4989-890e-aafb032f1e7d/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_56d6a4f4-5fa4-4989-890e-aafb032f1e7d.",
"origin_of": [],
"parts": [],
"pooled_in": [],
"release_timestamp": "2024-07-10T19:49:12.161536+00:00",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"term_name": "HCT116"
}
],
"schema_version": "23",
"sex": "male",
"sorted_fractions": [],
"sorted_from": {
"@id": "/in-vitro-systems/IGVFSM5118IDCI/",
"accession": "IGVFSM5118IDCI"
},
"sorted_from_detail": "FACS bin F for 80-100% expression of MYC",
"sources": [
{
"@id": "/sources/atcc/",
"title": "ATCC"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/",
"purpose": "activation",
"status": "in progress",
"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_type": "chemical"
}
],
"uuid": "06969354-ab6e-4338-aa07-8b741106281d",
"virtual": false
}