HG012053

Ian Whaling

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach

charles-gersbach

Charles Gersbach, Duke

Gregory Crawford

Tim Reddy

audit_item_status

audit_no_files

CRISPR FACS screen

CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

CRISPR activation FACS screen targeting IL2RA: alignments

CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications

STEMCELL Technologies

Alejandro Barrera

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Jurkat — IGVFSM2737ATVK

Attribution

Jurkat — IGVFSM2737ATVK

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Attribution