{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM2681JPHQ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2681JPHQ",
"age": "unknown",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep1-BinA"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM2681JPHQ](/in-vitro-systems/IGVFSM2681JPHQ/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM2681JPHQ/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing plasmid map",
"detail": "Construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/) does not have a plasmid map attached in `documents`. Construct library sets are expected to be associated with a plasmid map document.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_construct_library_set_plasmid_map",
"path": "/construct-library-sets/IGVFDS6377KNCH/"
},
{
"category": "missing guide RNA sequences",
"detail": "Construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/) has no `integrated_content_files`. Guide libraries are expected to link to an integrated content file of guide RNA sequences.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_integrated_content_files",
"path": "/construct-library-sets/IGVFDS6377KNCH/"
}
],
"WARNING": [
{
"category": "missing files",
"detail": "Construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/) has no `files`. File sets are expected to have files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_no_files",
"path": "/construct-library-sets/IGVFDS6377KNCH/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6377KNCH/",
"accession": "IGVFDS6377KNCH",
"file_set_type": "guide library",
"status": "in progress"
}
],
"creation_timestamp": "2023-11-30T22:30:36.102676+00:00",
"demultiplexed_to": [],
"donors": [
"/human-donors/IGVFDO1080XFGV/"
],
"file_sets": [
{
"@id": "/measurement-sets/IGVFDS1466VGTF/",
"accession": "IGVFDS1466VGTF",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep1-BinA_AmpliconSequencing"
],
"file_set_type": "experimental data",
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"preferred_assay_title": "Variant-EFFECTS",
"status": "released",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
{
"@id": "/analysis-sets/IGVFDS1863TJOE/",
"accession": "IGVFDS1863TJOE",
"aliases": [
"jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-Stimulation_Variant-FlowFISH_analysis"
],
"file_set_type": "principal analysis",
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "prime editing Variant-EFFECTS targeting PPIF: variant effects"
}
],
"institutional_certificates": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"cas": "nCas9",
"cas_species": "Streptococcus pyogenes (Sp)",
"fused_domain": "M-MLV RT (PE2)",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"multiplexed_in": [],
"notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\" This sample's modifications originally included Modification /modifications/e8d549e6-cef0-482a-9deb-76d3b7ecaac3/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_e8d549e6-cef0-482a-9deb-76d3b7ecaac3.",
"origin_of": [],
"parts": [],
"pooled_in": [],
"release_timestamp": "2023-12-20T22:54:33.626001+00:00",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"status": "released",
"term_name": "Jurkat"
}
],
"schema_version": "23",
"sex": "male",
"sorted_fractions": [],
"sorted_from": {
"@id": "/in-vitro-systems/IGVFSM3853IUEN/",
"accession": "IGVFSM3853IUEN",
"status": "released"
},
"sorted_from_detail": "FACS bin for 0-10% expression of PPIF",
"sources": [
{
"@id": "/sources/atcc/",
"status": "released",
"title": "ATCC"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/df02c0d4-f796-4f77-bfbc-388cdbfcc615/",
"depletion": false,
"purpose": "stimulation",
"status": "released",
"treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain",
"treatment_type": "protein"
},
{
"@id": "/treatments/42b3c61d-38de-4bd2-bb39-33c125a417cb/",
"depletion": false,
"purpose": "stimulation",
"status": "released",
"treatment_term_name": "phorbol 13-acetate 12-myristate",
"treatment_type": "chemical"
}
],
"uuid": "b4e90850-d1f6-468c-b726-6bb3d6917662",
"virtual": false
}