HCT116 — IGVFSM2608ULOG

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS1891GTLU guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM4608EHIU FACS bin F for 80-100% expression of FAM3C

Aliases

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR1-Rep1_S138

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR2-Rep1_S144

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR3-Rep1_S192

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR4-Rep1_S198

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS5239VQTN

CRISPR degradation, interference FlowFISH screen targeting FAM3C: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_FAM3C

Jesse Engreitz, Stanford

IGVFDS5772WTGS

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR1-Rep1_S138
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR2-Rep1_S144
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR3-Rep1_S192
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR4-Rep1_S198

Jesse Engreitz, Stanford

IGVFDS6901XNIT

mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR1-Rep1_S138
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR2-Rep1_S144
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR3-Rep1_S192
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinF-PCR4-Rep1_S198

Jesse Engreitz, Stanford

IGVFDS9190KTAF

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets