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HCT116 — IGVFSM2548FJWP

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
  • IGVFDS7857SYCK Guide (sgRNA) library targeting accessible genome regions in many genomic loci
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM5118IDCI FACS bin E for 80-90% expression of MYC
Aliases
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep1_S139
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep2_S194
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep1_S145
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep2_S200
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep1_S151
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep2_S206
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep1_S157
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep2_S212

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

3 items
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep1_S139
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep2_S194
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep1_S145
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep2_S200
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep1_S151
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep2_S206
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep1_S157
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep2_S212
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin6hrs-FF2
Jesse Engreitz, Stanford
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep1_S139
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep2_S194
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep1_S145
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep2_S200
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep1_S151
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep2_S206
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep1_S157
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep2_S212
Jesse Engreitz, Stanford

Modifications

2 items
degradation
interference

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford