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HCT116 — IGVFSM2476YZDG

released
Summary
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
  • IGVFDS5413RKIA guide (sgRNA) library targeting accessible genome regions in many genomic loci
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM5105OEUU FACS bin A for 0-10% expression of SSFA2
Aliases
jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR1-Rep1_S37
jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR2-Rep1_S43
jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR3-Rep1_S91
jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR4-Rep1_S97
Attribution
Jesse Engreitz, Stanford

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
CRISPR degradation, interference FlowFISH screen targeting ITPRID2: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_SSFA2
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin6hrs-FF2
Jesse Engreitz, Stanford
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR1-Rep1_S37
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR2-Rep1_S43
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR3-Rep1_S91
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR4-Rep1_S97
Jesse Engreitz, Stanford
gRNA sequencing
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR1-Rep1_S37
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR2-Rep1_S43
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR3-Rep1_S91
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR4-Rep1_S97
Jesse Engreitz, Stanford

Modifications

2 items
degradation
interference

Treatments

1 item
Term Name
Purpose
Type
Summary
activation
chemical
Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours

Documents