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THP-1 — IGVFSM2418RCAK

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This object is in preview status. Metadata is subject to change until this object is released.
Summary
Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
  • IGVFDS6555EOZU guide (sgRNA) library targeting sequence variants in a genomic locus
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM3187FGMR FACS bin for 51-75% expression of PPIF
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinC
Protocols
Attribution
Jesse Engreitz, Stanford

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_THP1
male
Homo sapiens

File Sets

2 items
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1_Variant-EFFECTS_analysis
Jesse Engreitz, Stanford
CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinC_AmpliconSequencing
Jesse Engreitz, Stanford

Modifications

1 item
Summary
Modality
Activation
prime editing