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Jurkat — IGVFSM2353FEES

released
Summary
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM1127DRPZ FACS bin for 80-90% expression of PPIF
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinE
Attribution
Jesse Engreitz, Stanford

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

File Sets

2 items
CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinE_AmpliconSequencing
Jesse Engreitz, Stanford
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-NoStimulation_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford

Modifications

1 item
Summary
Modality
Activation
prime editing