HCT116 — IGVFSM2025NTWT

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM2423BKRB FACS bin C for 35-45% expression of CCND1

Aliases

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR1-Rep1_S243

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR2-Rep1_S249

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR3-Rep1_S285

jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR4-Rep1_S291

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS1569RNPD

CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_CCND1

Jesse Engreitz, Stanford

IGVFDS3120IRAP

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR1-Rep1_S243
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR2-Rep1_S249
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR3-Rep1_S285
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR4-Rep1_S291

Jesse Engreitz, Stanford

IGVFDS6842FAIJ

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets