HCT116 — IGVFSM1737WNTH

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM4717ECVY FACS bin F for 80-100% expression of KITLG

Aliases

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR1_S18

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR2_S24

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR3_S114

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR4_S120

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS0426GXQB

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR1_S18
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR2_S24
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR3_S114
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR4_S120

Jesse Engreitz, Stanford

IGVFDS4522DABT

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets