{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM1725FTKZ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1725FTKZ",
"age": "unknown",
"aliases": [
"jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR1-Rep1_S13",
"jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR2-Rep1_S19",
"jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR3-Rep1_S67",
"jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR4-Rep1_S73"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"classifications": [
"cell line"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS5413RKIA/"
],
"creation_timestamp": "2024-05-14T19:57:06.805836+00:00",
"demultiplexed_to": [],
"documents": [
"/documents/a1a19741-90cc-46ea-b90c-96c4a4b70d8e/",
"/documents/b0663905-6249-42ea-9937-9b2f645c787d/"
],
"donors": [
"/human-donors/IGVFDO0384OZRW/"
],
"file_sets": [
{
"@id": "/auxiliary-sets/IGVFDS0973WJFG/",
"accession": "IGVFDS0973WJFG",
"aliases": [
"jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR1-Rep1_S13",
"jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR2-Rep1_S19",
"jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR3-Rep1_S67",
"jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR4-Rep1_S73"
],
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS9987GPMG/",
"accession": "IGVFDS9987GPMG",
"aliases": [
"jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR1-Rep1_S13",
"jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR2-Rep1_S19",
"jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR3-Rep1_S67",
"jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR4-Rep1_S73"
],
"assay_term": {
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
{
"@id": "/auxiliary-sets/IGVFDS4597CMXX/",
"accession": "IGVFDS4597CMXX",
"aliases": [
"jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin0hrs-FF2"
],
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "cell sorting for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets"
}
],
"institutional_certificates": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"modifications": [
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"multiplexed_in": [],
"notes": "This sample's modifications originally included Modification /modifications/56d6a4f4-5fa4-4989-890e-aafb032f1e7d/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_56d6a4f4-5fa4-4989-890e-aafb032f1e7d.",
"origin_of": [],
"parts": [],
"pooled_in": [],
"release_timestamp": "2024-07-10T19:51:31.425674+00:00",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"term_name": "HCT116"
}
],
"schema_version": "23",
"sex": "male",
"sorted_fractions": [],
"sorted_from": {
"@id": "/in-vitro-systems/IGVFSM5418TMHK/",
"accession": "IGVFSM5418TMHK"
},
"sorted_from_detail": "FACS bin A for 0-10% expression of SSFA2",
"sources": [
{
"@id": "/sources/atcc/",
"title": "ATCC"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) modified with a guide library",
"taxa": "Homo sapiens",
"uuid": "edb9dc0b-0681-4acd-b913-c050e424de1a",
"virtual": false
}