HCT116 — IGVFSM1617PVUY

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM3580KDPA FACS bin E for 80-90% expression of CCND1

Aliases

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR1_S77

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR2_S173

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR3_S269

jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR4_S365

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS2726RPEW

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR1_S77
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR2_S173
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR3_S269
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR4_S365

Jesse Engreitz, Stanford

IGVFDS2982SJGW

CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_CCND1_pilot

Jesse Engreitz, Stanford

IGVFDS3741DKGN

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR1_S77
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR2_S173
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR3_S269
jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR4_S365

Jesse Engreitz, Stanford

IGVFDS5292OCBC

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets