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HCT116 — IGVFSM1436SJKL
HCT116 — IGVFSM1436SJKL
released
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Summary
HCT116 cell line, male, Homo sapiens modified with a guide library
Sample Terms
HCT116
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
IGVFDS0392OWNZ
Guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
Taxa
Homo sapiens
Sources
atcc
Aliases
jesse-engreitz:in-vitro-system_CRUDO_PILOT
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO0384OZRW
igvf:donor_of_HCT116
male
Homo sapiens
Origin Sample Of
2 items
Accession
Type
Sample Terms
Summary
IGVFSM3580KDPA
InVitroSystem
HCT116
HCT116 cell line, male, Homo sapiens modified with a guide library
IGVFSM6219XAXK
InVitroSystem
HCT116
HCT116 cell line, male, Homo sapiens treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with a guide library
Modifications
1 item
Summary
Modality
CRISPRi Sp-dCas9-KOX1-KRAB
interference
Documents
2 items
Page
Description
Type
Download
Preview
Generation of inducible CRISPRi line
plasmid map
addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Download addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Download addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Generation of inducible CRISPRi line
plasmid map
takara#631363_pLVX-EF1alpha-Tet3G.pdf
Download takara#631363_pLVX-EF1alpha-Tet3G.pdf
Download takara#631363_pLVX-EF1alpha-Tet3G.pdf
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford