GM25256 (WTC-11) — IGVFSM1288TQKF

preview

This object is in preview status. Metadata is subject to change until this object is released.

Summary

GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW282), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Sample Terms

GM25256 (WTC-11)

Classification

differentiated cell specimen

Sex

male

Cellular Sub Pool

subpool_LW282

Originated From Sample

IGVFSM6456YEWR

Age

unknown

Part of Sample

IGVFSM9036LWJT

Time Post Change

20 day

Cell Fate Change Protocol

protocol_NPC_Differentiation_Munshi_lab_1.pdf

Construct Library Sets

IGVFDS2302LCEO guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Description

differentiated WTC11 -dCAS9-KRAB-Lenti-sgRNA

Sources

Nikhil Munshi, UT

Submitter Comment

DIfferentiated WTC11 cell line (Lenti)

Aliases

nikhil-munshi:differentited_WTC11_TF_Perturb_Seq_Pilot_NPC_Lenti_LW282

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO1756PPKO

igvf:donor_of_WTC11

male

Homo sapiens

File Sets

4 items

IGVFDS1588TVKF

interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix

gary-hon:WTC11_TF_Perturb_Seq_Pilot_NPC_Lenti_PrimaryAnalysis

Gary Hon, UT

IGVFDS3567LQMB

cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci