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HCT116 — IGVFSM1124JJAA

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM6328ZXHI FACS bin E for 80-90% expression of MYC
Aliases
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep1_S29
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep2_S84
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep1_S35
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep2_S90
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep1_S41
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep2_S96
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep1_S47
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep2_S102

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep1_S29
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep2_S84
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep1_S35
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep2_S90
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep1_S41
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep2_S96
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep1_S47
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep2_S102
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting MYC: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_MYC
Jesse Engreitz, Stanford
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep1_S29
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep2_S84
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep1_S35
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep2_S90
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep1_S41
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep2_S96
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep1_S47
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep2_S102
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin0hrs-FF2
Jesse Engreitz, Stanford

Modifications

2 items
interference
degradation

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford