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HCT116 — IGVFSM0904FNLS

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM6219XAXK FACS bin F for 80-100% expression of CCND1
Aliases
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR1_S6
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR2_S102
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR3_S198
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR4_S294

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR1_S6
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR2_S102
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR3_S198
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR4_S294
Jesse Engreitz, Stanford
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR1_S6
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR2_S102
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR3_S198
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR4_S294
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting CCND1: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_CCND1_pilot
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_PILOT_CCND1-Auxin6hrs-FF1
Jesse Engreitz, Stanford

Modifications

2 items
degradation
interference

Treatments

1 item
Term Name
Purpose
Type
Summary
activation
chemical
Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford