{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM0841TQDY/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0841TQDY",
"age": "unknown",
"aliases": [
"jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinA"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM0841TQDY](/in-vitro-systems/IGVFSM0841TQDY/) has in progress subobject construct library set [IGVFDS2374NXLW](/construct-library-sets/IGVFDS2374NXLW/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM0841TQDY/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"classifications": [
"cell line"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS2374NXLW/"
],
"creation_timestamp": "2023-11-30T22:29:48.599806+00:00",
"demultiplexed_to": [],
"donors": [
"/human-donors/IGVFDO7359LVPI/"
],
"file_sets": [
{
"@id": "/measurement-sets/IGVFDS8504HJPW/",
"accession": "IGVFDS8504HJPW",
"aliases": [
"jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinA_AmpliconSequencing"
],
"assay_term": {
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "Variant FlowFISH integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
{
"@id": "/analysis-sets/IGVFDS5031MNRR/",
"accession": "IGVFDS5031MNRR",
"aliases": [
"jesse-engreitz:PPIF_enhancer_tiling_THP1_Variant-FlowFISH_analysis"
],
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "released",
"summary": "principal analysis of Variant FlowFISH data"
},
{
"@id": "/auxiliary-sets/IGVFDS4645DCQC/",
"accession": "IGVFDS4645DCQC",
"aliases": [
"jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinA_AmpliconSequencing_AuxiliarySet"
],
"lab": {
"title": "Jesse Engreitz, Stanford"
},
"status": "in progress",
"summary": "variant sequencing for Variant FlowFISH integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"institutional_certificates": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"multiplexed_in": [],
"notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\" This sample's modifications originally included Modification /modifications/e8d549e6-cef0-482a-9deb-76d3b7ecaac3/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_e8d549e6-cef0-482a-9deb-76d3b7ecaac3.",
"origin_of": [],
"parts": [],
"pooled_in": [],
"release_timestamp": "2023-12-20T22:56:41.626095+00:00",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"term_name": "THP-1"
}
],
"schema_version": "23",
"sex": "male",
"sorted_fractions": [],
"sorted_from": {
"@id": "/in-vitro-systems/IGVFSM0481XUYL/",
"accession": "IGVFSM0481XUYL"
},
"sorted_from_detail": "FACS bin for 0-25% expression of PPIF",
"sources": [
{
"@id": "/sources/atcc/",
"title": "ATCC"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with a guide library",
"taxa": "Homo sapiens",
"uuid": "e387ebf2-3a2a-4a69-bad8-729c1fa0bd29",
"virtual": false
}