HCT116 — IGVFSM0811AZWF

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS7846UISG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM0637ZUKN FACS bin C for 35-45% expression of KITLG

Aliases

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR1_S27

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR2_S33

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR3_S123

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR4_S129

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS0329USVJ

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin0hrs-FF3

Jesse Engreitz, Stanford

IGVFDS1862OQFF

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR1_S27
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR2_S33
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR3_S123
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR4_S129

Jesse Engreitz, Stanford

IGVFDS3143OSQG

mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR1_S27
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR2_S33
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR3_S123
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR4_S129

Jesse Engreitz, Stanford

IGVFDS6482AXWW

CRISPR degradation, interference FlowFISH screen targeting KITLG: differential element quantifications