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GM25256 (WTC-11) — IGVFSM0559UNMK

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Summary
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Sample Terms
Classification
differentiated cell specimen
Sex
male
Cellular Sub Pool
subpool_LW279
Originated From Sample
IGVFSM6456YEWR
Age
unknown
Part of Sample
IGVFSM6351SIZQ
Targeted Sample Term
cardiac muscle cell
Time Post Change
12 day
Cell Fate Change Protocol
Protocol-hPSC-CM-differentiation-protocol-DRAFTv2.1.pdf
Construct Library Sets
  • IGVFDS2302LCEO guide (sgRNA) library targeting accessible genome regions in many genomic loci
Taxa
Homo sapiens
Description
differentiated WTC11 -dCAS9-KRAB-Lenti-sgRNA
Sources
Nikhil Munshi, UT
Submitter Comment
DIfferentiated WTC11 cell line (Lenti)
Aliases
nikhil-munshi:differentited_WTC11_TF_Perturb_Seq_Pilot_CMs_Lenti_LW279
Attribution
Gary Hon, UT

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

File Sets

4 items
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_sgRNA_LW279
Gary Hon, UT
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_PrimaryAnalysis
Gary Hon, UT
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_HTO_LW279
Gary Hon, UT
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_LW279
Gary Hon, UT

Modifications

1 item
Summary
Modality
Activation
interference

Documents

1 item
Page
Description
Type
Download
Standardized File Format
Preview
Stem Cell DIfferentiation Protocol
cell fate change protocol