HCT116 — IGVFSM0427NNRS

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS5413RKIA guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM5105OEUU FACS bin B for 10-20% expression of SSFA2

Aliases

jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR1-Rep1_S38

jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR2-Rep1_S44

jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR3-Rep1_S92

jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR4-Rep1_S98

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS2031CTSP

CRISPR degradation, interference FlowFISH screen targeting ITPRID2: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_SSFA2

Jesse Engreitz, Stanford

IGVFDS2912UBNW

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR1-Rep1_S38
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR2-Rep1_S44
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR3-Rep1_S92
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR4-Rep1_S98

Jesse Engreitz, Stanford

IGVFDS3809LQRK

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin6hrs-FF2

Jesse Engreitz, Stanford

IGVFDS4798YRBK

mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR1-Rep1_S38
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR2-Rep1_S44
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR3-Rep1_S92
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR4-Rep1_S98

Jesse Engreitz, Stanford