THP-1 — IGVFSM0397MOBU

preview

This object is in preview status. Metadata is subject to change until this object is released.

Summary

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Sample Terms

THP-1

Classification

cell line

Sex

male

Age

unknown

Part of Sample

IGVFSM3448CJIW

Taxa

Homo sapiens

Sources

atcc

Aliases

jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_CAAC-THP1-BioRep2-FFrep1-FACS_Input

Protocols

https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO7359LVPI

igvf:donor_of_THP1

male

Homo sapiens

File Sets

2 items

IGVFDS1132MKHT

prime editing Variant-EFFECTS targeting PPIF: variant effects

jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_CAAC-THP1_Variant-EFFECTS_analysis

Jesse Engreitz, Stanford

IGVFDS3465JQXG

CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus

jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_CAAC-THP1-BioRep2-FFrep1-FACS_Input_AmpliconSequencing

Jesse Engreitz, Stanford

Modifications

1 item

Summary

Modality

CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

prime editing

Attribution

Award

HG011972

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Jesse Engreitz

Lab

Jesse Engreitz, Stanford