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HCT116 — IGVFSM0264XNND

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM5634GTNX FACS bin E for 80-90% expression of MYC
Aliases
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR1_Rep1_S115
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR1_Rep2_S170
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR2_Rep1_S121
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR2_Rep2_S176
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR3_Rep1_S127
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR3_Rep2_S182
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR4_Rep1_S133
jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR4_Rep2_S188

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR1_Rep1_S115
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR1_Rep2_S170
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR2_Rep1_S121
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR2_Rep2_S176
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR3_Rep1_S127
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR3_Rep2_S182
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR4_Rep1_S133
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR4_Rep2_S188
Jesse Engreitz, Stanford
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR1_Rep1_S115
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR1_Rep2_S170
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR2_Rep1_S121
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR2_Rep2_S176
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR3_Rep1_S127
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR3_Rep2_S182
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR4_Rep1_S133
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinE-PCR4_Rep2_S188
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting MYC: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_MYC
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin6hrs-FF1
Jesse Engreitz, Stanford

Modifications

2 items
degradation
interference

Treatments

1 item
Term Name
Purpose
Type
Summary
activation
chemical
Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford