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Jurkat — IGVFSM0193KFOU

released
Summary
Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Part of Sample
IGVFSM3853IUEN
Taxa
Homo sapiens
Sources
atcc
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep1-FACS_Input

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

File Sets

2 items
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep1-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-Stimulation_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford

Modifications

1 item
Summary
Modality
prime editing

Treatments

2 items
Summary
stimulation
chemical
Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours
stimulation
protein
Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford