CRISPRa Sa-dCas9-2xVP64

released

Summary

Modality

activation

Cas

dCas9

Cas Species

Staphylococcus aureus (Sa)

Fused Domain

2xVP64

Sources

addgene addgene:194279

Aliases

charles-gersbach:dcas9-crispra-2x-vp64

igvf:replacement_of_8c0f2c0a-c2c0-4d14-98fc-e1551c72667c

Submitter Comment

This Crispr Modification is an identical replacement of the previously submitted Modification 8c0f2c0a-c2c0-4d14-98fc-e1551c72667c.

Biosamples Modified by this Modification

9 items

Sample Terms

Summary

IGVFSM1838CFFU

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM2429HKWG

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM3244YHOI

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM4817IZZH

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM5112IFQB

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM6178KGGZ

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM6606RDWT

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM6721RTTV

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM6964ODEE

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)