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IGVFDS9869EMNE

released
File Set Type
guide library
Summary
guide (sgRNA) library targeting accessible genome regions in 29 genomic loci
Sources
Aliases
jesse-engreitz:construct_library_CRUDO_TAPseq

Guide Library Details

Library Construct Scope
loci
Selection Criteria
accessible genome regions
Small Scale Loci List
GRCh38 chr11 69352659-69353159, GRCh38 chr11 69636212-69636869, GRCh38 chr11 69637976-69639354, GRCh38 chr11 69640512-69641680, GRCh38 chr2 181784494-181785367, GRCh38 chr2 181790595-181792502, GRCh38 chr2 181810528-181811596, GRCh38 chr2 181876127-181877048, GRCh38 chr2 181891240-181893096, GRCh38 chr7 121298480-121299196
Guide Type
sgRNA
Tiling Modality
sparse peaks

Sequencing Results

1 item
Accession
File Format
Content Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
83 MB
Jesse Engreitz, Stanford
validated

Integrated Content Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
guide RNA sequences
GRCh38 guide RNA sequences
Jesse Engreitz, Stanford
5.3 KB
validated

Applied to Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Documents

1 item
Page
Description
Type
Download
Preview
Guide plasmid for TAP-seq
plasmid map

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford